| Contents of lectures | Morphological, physiological and biochemical characteristics of different groups of microorganisms (viruses, bacteria, yeasts, molds, mushrooms, algae, protozoa), microbial genetics (organization of genetic material, replication, transcription, translation, mutation, exchange of genetic material, basics of genetic engineering), factors necessary for the growth and reproduction of microorganisms in vitro condition, pathogenic microorganisms and their virulence factors, antimicrobial drugs (antibacterial, antifungal, antiviral, antiparasitic) and molecular mechanisms of their action, disinfectants, antiseptics and preservatives used in the pharmaceutical industry, factors affecting their activity and mechanisms of action, origin and mechanisms of acquiring resistance to antimicrobial agents and pathways for the spread of resistance through the microorganisms population, methods by which the growth of microorganisms can be controlled (mechanical, physical, chemical and biological methods), the effects of immunization (mechanisms of innate and acquired immunity) on infections caused by extracellular or intracellular microorganisms and types of vaccines (live attenuated, inactivated and recombinant vaccines), types of microorganisms which are used in the production of various pharmaceutical products (antibiotics, vitamins, amino acids, organic acids, enzymes), characteristics of probiotic strains and their application, the types of microorganisms which are most commonly causes... |
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| Contents of exercises | Examination of morphological characteristics of bacteria, yeasts, molds, algae and protozoa (preparation of wet-mount and hanging drop slide); simple, differential and specific structures staining of fixed preparations; microscopic measurement of microorganisms; methods for isolation of pure cultures, enumeration of microorganisms (indirect and direct method); examination of physiological characteristics of microorganisms; testing of biochemical characteristics of microorganisms; testing of antimicrobial activity of drugs, disinfectants, antiseptics and preservatives by agar diffusion method; determination of minimum inhibitory (MIC) and minimal lethal concentration (MLC) of antimicrobial agents; microbiological analysis of water; microbiological quality control of pharmaceutical products |
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