MHMS - Mass spectrometry

Course specification
Course titleMass spectrometry
Study programme
Lecturer (for classes)
Lecturer/Associate (for practice)
Lecturer/Associate (for OTC)
    ConditionОблик условљености
    The goalThe aim of the course is to show the great potential of mass spectrometry (MS) for the identification and quantification of organic compounds. In this regard special emphasis is placed on combined techniques, "GC-MS" and "LC-MS", which enable the analysis of complex mixtures as well as "ICP-MS" method and elemental isotopic analysis.
    The outcomeDuring the course, students should acquire knowledge that enables them to apply mass spectrometry in different areas of the structure analysis and identification of compounds in the quantitative determination of trace analytes in complex mixtures. Also, students will develop experimental skills in the application of combined techniques "NRLC-MS", "HPLC-MS/MS" and "ICP-MS".
    Contents of lecturesThe program includes traditional activities, explaining the basic principles of mass spectrometry, as well as recent developments, particularly in the field of ionization techniques (EI, CI, APCI, ESI, MALDI ) and of different types of analyzers ( magnetic, electrostatic, quadrupole, different types of ion alignment, analyzer based on time of flight). The characteristics of different combinations of analyzers in hybrid instruments are included, which allow significantly expand the application of mass spectrometry as a method of chemical analysis, as well as a research discipline. For the identification of organic compounds using ion fragmentation reactions, the so-called "MS -MS" spectra for quantitative analysis of complex mixtures using the combined techniques "GC/MS" or "HPLC/MS" or "HPLC/MS- MS" will be demonstrated in the laboratory. Special attention will be paid to izotopic analysis and quantitative analysis of metals.
    Contents of exercisesLabs: Recording mass spectra of compounds and the isotopic composition of elements, tandem mass spectrometry, liquid chromatography with mass spectrometric detection, coupled plasma with mass spectrometric detection.
    1. B. Ardrey, Liquid Chromatography – Mass Spectrometry: An Introduction, John Wiley and Sons, Chichester, 2003.
    2. R.M. Smith and K.L. Busch, Understanding Mass Spectra – A Basic Approach, John Wiley and Sons, New York, 1999.
    3. J. Barker, Mass Spectrometry, John Wiley and Sons, Chichester, 1999.
    4. T.A. Lee, A Beginner's Guide to Mass Spectral Interpretation, John Wilea and Sons, Chichester, 1998.
    Number of hours per week during the semester/trimester/year
    LecturesExercisesOTCStudy and ResearchOther classes
    Methods of teaching
    Knowledge score (maximum points 100)
    Pre obligationsPointsFinal examPoints
    Activites during lecturesTest paper50
    Practical lessons20Oral examination