D103 - Biochemical kinetics

Course specification
Course titleBiochemical kinetics
Study programme
Lecturer (for classes)
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ConditionnoneОблик условљености
      The goalThe aim of the course is to teach the students specialized and novel trends and achievements in the area of biochemical kinetics as well as provide experience in modern tools and techniques of kinetic analysis based on selected examples from the scientific literature by using software packages specialized for biochemical reactions. The aim is also to provide the students competences to critically analyze the relevance of the kinetic models, and how they can be used in a practical fashion to design and optimize biochemical processes.
      The outcome(i) Students extended their knowledge and gained skill to analyze complex problems in the area of biochemical kinetics which are necessary for design and optimization of biochemical processes; (ii) Students gained skills and knowledge to critically analyze kinetic data as well as to independently adapt optimal kinetic models for describing complex biochemical reactions in different systems; (iii) Students gained technical competencies to use software packages specialized for biochemical reactions (Matematica, EZ-FIT V1.1, ENCORA 1.2); (iv) Students gained competencies to use specialized knowledge for critical analysis and evaluation of the adequacy of the corresponding models in specific cases and on that basis to make strategic decisions; (v) Students gained communication and social competencies required for work in a team of engineers. ;
      Contents of lecturesThis course will explore the theoretical and practical aspects of both enzyme and microbial kinetics. Topics covered include: biochemical thermodynamics, novel tools and techniques of kinetic analysis, kinetic description of reversible and two-substrate enzyme reactions, pH and temperature dependence of enzyme-catalyzed reactions, kinetic treatment of multisite and cooperative enzymes (sequential interaction model and symmetry model), putting kinetic principles into practice, use of enzyme kinetic data in the study of structure-function relationships of proteins. Topics from microbial process include kinetic description of microbial growth in complex systems, mixed substrate utilization and microbial competition and kinetics of multiple-nutrient-controlled growth.
      Contents of exercisesThe practical part consists of exercises and computer lab. Exercises with two-substrate enzyme kinetics; Exercises with thermodynamics, competition and growth of mixed cultures. Research journal articles will be used as case studies to illustrate the practical application of enzyme and microbial kinetics. Emphasis will be placed on basic principles as well as hands-on experiences in using software tools to solve practical problems (Matematica, EZ-FIT V1.1, ENCORA 1.2).
      1. R.A. Alberty, Enzyme kinetics: rapid-equilibrium applications of Matematica, Wiley, 2011.
      2. A.G. Marangoni, Enzyme kinetics: a modern approach, John Wiley & Sons, 2003.
      3. Z. Knežević-Jugović, “Enzimsko inženjerstvo”, Tehnološko-metalurški fakultet, Beograd, 2008.
      4. V. Leskovac, Comprehensive enzyme kinetics, Springer, 2003.
      5. D.L. Purich, R.D.Allison, Handbook of biochemical kinetics: a guide to dynamic processes in the molecular life sciences, Academic Press, 1999.
      Number of hours per week during the semester/trimester/year
      LecturesExercisesOTCStudy and ResearchOther classes
      Methods of teachingThe course consists of lectures, exercises, computer simulations.
      Knowledge score (maximum points 100)
      Pre obligationsPointsFinal examPoints
      Activites during lectures15Test paper50
      Practical lessonsOral examination