14MEB - Electrochemical Biosensors

Course specification
Course title		Electrochemical Biosensors
Acronym		14MEB
Study programme		Biochemical Engineering and Biotechnology,Chemical engineering
Module
Lecturer (for classes)		prof. dr Zorica Knežević-Jugović prof. dr Milica Gvozdenović
Lecturer/Associate (for practice)		prof. dr Zorica Knežević-Jugović prof. dr Milica Gvozdenović
Lecturer/Associate (for OTC)
ESPB		4.0	Status
Condition		none	Облик условљености
The goal		To provide students basic knowledge related to operational principles of electrochemical biosensors, with emphasis on practical application in food chemistry, analysis of biological contaminants and application in pharmaceutical industry.
The outcome		Students would adopt knowledge on operational principles of electrochemical biosensors and their application. They would be introduced to development of the specific immobilization techniques of receptor components of the biosensor and basic parameters influencing biosensor function. Student would also be introduced to classification of electrochemical sensors (potentiometric, amperometric, conductometric and impedimetric). They would understand the term and means of formation of enzyme electrodes and be informed on contemporary trends in application of newly developed materials for the fabrication of enzyme electrodes.
Contents
Contents of lectures		Operational principles of electrochemical biosensors. Major properties (selectivity, range, the nature of the output signal, repeatability and reproducibility, response time, storage time) Enzymes in biosensors, basic structural characteristics. The methods of immobilization of the biomaterial for receptor component (enzymes, antibodies, cells). The influence of the immobilization parameters and techniques on electrochemical biosensors. Novel trends in immobilization and application of: membranes, mediators, modified electrodes for improvement of the electrochemical biosensors function. Mono enzime sensors and sensors with coupled enzymes. Selectivity and possible interference with other bioactive components. Classification of the electrochemical biosensors based on nature of the output signal. Enzyme and non enzyme sensors. Enzyme electrodes. Electrochemical biosensors based on antibodies. The development of microbe sensors and their application. Trends in development of nanostructured materials for fabrication of sensors electrodes. ; Practical aspects of electrochemical biosensors: detection of pesticides, glucose, hydrogen peroxide, urea, ascorbic acid, immuno-sensors. ;
Contents of exercises		Immobilization of enzymes onto materials used in fabrication of sensor electrodes and efficeiency of the immobilization ; The influence of electrode pretreatment on quality of the output signal of non enzymatic electrochemical biosensor; ; Electrochemical determination of ascorbic acid ; Determination of the enzyme kinetic parameters (ex. enzyme electrode for determination of glucose). ;
Literature
“Biosensors“, Ed. P. A. Sierra, Intech, Vukovar, 2010. F. Scheller, F. Schubert, „Biosensors“, Elsevier Science Publishers B.V., Amsterdam, 1992. “Electrochemical Sensors Biosensors and Their Biomedical Application“, Ed. X. Zang, H. Ju, J. Wang, Academic Press, Elsevier, 2008. J. Wang, “Analytical Electrochemistry” 3rd ed. John Wiley & Sons, Inc. Hoboken, New Jersey, 2006.
Number of hours per week during the semester/trimester/year
Lectures	Exercises	OTC	Study and Research	Other classes
2	1	1
Methods of teaching		Lectures (PowerPoint presentations) and laboratory experiments. Consultations, seminars encompassing relevant literature and discussion of the selected problems.
Knowledge score (maximum points 100)
Pre obligations		Points	Final exam	Points
Activites during lectures			Test paper	50
Practical lessons		10	Oral examination
Projects
Colloquia		20
Seminars		20