14MBIB12 - Molecular Diagnostics

Course specification
Course title		Molecular Diagnostics
Acronym		14MBIB12
Study programme		Biochemical Engineering and Biotechnology
Module
Lecturer (for classes)		prof. dr Mirjana Rajilić-Stojanović
Lecturer/Associate (for practice)		prof. dr Mirjana Rajilić-Stojanović
Lecturer/Associate (for OTC)
ESPB		4.0	Status
Condition		Microbiology, Biochemistry	Облик условљености
The goal		To gain the knowledge about identification of microorganisms using genetic information and molecular techniques.
The outcome		Theoretical knowledge about the molecular basis for the systematics of organism, particularly those base don the sequence of the 16/18 S rRNA gene, and use of this sequence in the rapid identification of organisms. Theoretical and practical knowledge about molecular techniques that can be used for the analysis of complex microbial ecosystems that can be found in food, water and other biological materials.
Contents
Contents of lectures		Theoretical knowledge about sysetematics of organisms based on the DNA sequence, and the use of molecular techniques for the analysis of (complex) microbial ecosystems. The main focus of the subject is understanding principles and interpretation of results obtained with the following molecular techniques: isolation of nucleic acids, polymerase chain reaction (PCR), ligation detection reaction (LDR), hybridization of DNA molecules with oligonucleotide probes both on the level of cells, or on the DNA array platforms, molecular techniques for profiling such as denaturant gradient gel electrophoresis (DGGE). The subject will give basis for bioinformatics analysis that include searches in the public databases, analysis and identification of organisms based on the DNA sequence.
Contents of exercises		Practical part of the subject will include laboratory projects, where students will apply some of the molecular techniques that will be applied for the diagnostics of model ecosystems. Bioinformatics exercises will include analysis of the 16S or 18S rRNA sequence using software such as ClustalX and ARB and searches of the public databases.
Literature
Prescott, L. M., J. P. Harley, and D. A. Klein. 2005. Microbiology Mc Graw Hill Higher Education ISBN: 978-0-07-255678-0 K. Zengler Accessing Uncultivated Microorganisms: from the Environment to Organisms and Genomes and Back 2008 . ASM Press, Herndon, VA; 2008 ISBN: 978-1-55581-406-9 M. Osborn, C Smith Molecular Microbial Ecology (Advanced Methods) 2007 Taylor & Francis ISBN-10: 1859962831 Lecutre notes
Number of hours per week during the semester/trimester/year
Lectures	Exercises	OTC	Study and Research	Other classes
1	1	1
Methods of teaching		Lectures, practical work (laboratory and bioinformatics exercises), essay.
Knowledge score (maximum points 100)
Pre obligations		Points	Final exam	Points
Activites during lectures			Test paper
Practical lessons		20	Oral examination	60
Projects
Colloquia
Seminars		20