14DIZ8 - Liquid Chromatography - Mass Spectrometry

Course specification
Course titleLiquid Chromatography - Mass Spectrometry
Study programme
Lecturer (for classes)
Lecturer/Associate (for practice)
Lecturer/Associate (for OTC)
    ConditionОблик условљености
    The goalThe overall goals of the course are to introduce students to the basic principles of high performance liquid chromatography - mass spectrometry and the application of this method for the analysis of environmental, food and pharmaceutical samples. Through practical work in the laboratory, students should acquire basic skills to work on LC-MS instrument. By completing essay, related to their area of interest, students should learn all aspects of the development of a modern analytical method.
    The outcomeStudents gain knowledge related to the principle of operation of liquid chromatograph - mass spectrometer and basic skills to work on the instrument; developing creativity and the ability to use acquired knowledge in solving actual analytical problems; through written and oral seminar presentation they should expand the communication skills.
    Contents of lecturesIntroduction to chromatography. High performance liquid chromatography. Mass spectrometry. Ionization techniques under atmospheric pressure. Tandem mass spectrometry. Method development. Sample preparation. Interpretation of mass spectra. Quantitative Analysis. Examples from practice.
    Contents of exercisesThe development of chromatographic method. ; The principle of operation of the mass spectrometer, instrument tuning. ; Interpretation of mass spectra. ; Setting instrumental method and method for data processing. ; Sample preparation for analysis. ; Analysis of real samples by liquid chromatography-mass spectrometry.
    1. Lecture notes and laboratory manual
    2. Ardrey R. E., Liquid Chromatography-Mass Spectrometry, John Wiley&Sons Ltd, Chichester, England, 2003. ;
    3. Niessen W.M.A:, Liquid Chromatography-Mass Spectrometry, CRC Press, Boca Raton, 2006.
    4. Cole, R.B., Electrospray Ionization Mass Spectrometry, John Wiley&Sons INC, New York, 1997.
    5. Relevant scientific journals
    Number of hours per week during the semester/trimester/year
    LecturesExercisesOTCStudy and ResearchOther classes
    Methods of teachinglectures, laboratory exercises, consultations
    Knowledge score (maximum points 100)
    Pre obligationsPointsFinal examPoints
    Activites during lecturesTest paper
    Practical lessonsOral examination50