14D141 - Mass spectrometry

Course specification
Course titleMass spectrometry
Study programme
Lecturer (for classes)
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ConditionОблик условљености
      The goalThe aim of the course is to show the great potential of mass spectrometry (MS) for the identification and quantification of organic compounds. In this respect, besides the usual topics on ionization and mass analysis, the combined instruments "GC-MS" and "LC-MS", which enable the analysis of complex mixtures are presented.
      The outcomeDuring the course, students should acquire the knowledge that enables them to apply mass spectrometry in different areas of structural analysis, identification of the compounds and quantitative determination of trace analytes in complicated mixtures. Students will develop the experimental skills in the application of combined techniques "HPLC-MS", "HPLC-MS/MS" and "ICP-MS".
      Contents of lecturesThe program includes traditional approach, explaining the basic principles of mass spectrometry, as well as recent developments, particularly in the field of ionization techniques (EI, CI, APCI, ESI, MALDI) and different types of analyzers (magnetic, electrostatic, quadrupole, different types of ion traps, analyzer based on time of flight). Included are the characteristics of different hybrid instruments, which significantly expand the application of mass spectrometry as a method of chemical analysis. For the identification of organic compounds using collision induced reactions, "MS-MS" spectra will be explained as well as the combined techniques "GC/MS", "GC/MS-MS", "HPLC/MS" and "HPLC/MS-MS”.
      Contents of exercisesLabs: Recording mass spectra of compounds and the isotopic composition of elements (ICP-MS), tandem mass spectrometry "HPLC / MS" and "HPLC / MS-MS" will be demonstrated in the laboratory.
      1. B. Ardrey, Liquid Chromatography – Mass Spectrometry: An Introduction, John Wiley and Sons, Chichester, 2003.
      2. R.M. Smith and K.L. Busch, Understanding Mass Spectra – A Basic Approach, John Wiley and Sons, New York, 1999.
      3. J. Barker, Mass Spectrometry, John Wiley and Sons, Chichester, 1999.
      4. T.A. Lee, A Beginner's Guide to Mass Spectral Interpretation, John Wilea and Sons, Chichester, 1998.
      Number of hours per week during the semester/trimester/year
      LecturesExercisesOTCStudy and ResearchOther classes
      Methods of teachingThe classes are held through the lectures, consultations with supervisor on selected topic, individual visits to the laboratory where the applied method is used, the presentation of the seminar paper and team preparation of the learning materials. At the end of the course, each student takes a test.
      Knowledge score (maximum points 100)
      Pre obligationsPointsFinal examPoints
      Activites during lecturesTest paper50
      Practical lessons20Oral examination