MBIB02 - Selected topics in biochemical engineering

Course specification
Course titleSelected topics in biochemical engineering
Study programme
Lecturer (for classes)
Lecturer/Associate (for practice)
Lecturer/Associate (for OTC)
    ConditionОблик условљености
    The goalThe aim of the course is to give the students more fundamentals and practical knowledge about general rules for optimization, modeling and design of enzymatic and other bioprocesses in non-conventional media. Based on selected topics from pharmaceutical industry students will be able to implement both biochemical and engineering knowledge in order to design new and improve current biotechnological processes.
    The outcomeAfter the course students will gain knowledge of basic principle of biocatalysis in actual biotechnological and pharmaceutical processes in non-conventional media. They will be able to apply the theoretical knowledge to specific industrial bioprocess, predict the course of a biochemical process by kinetic calculation and design new processes in these complex systems.
    Contents of lectures Kinetics of two-substrate reactions. Progress curve and determination of optimal kinetic models. The inhibition of two-substrate reactions: kinetic models. Mechanisms of drug actions by enzyme inhibition. ;  Biocatalysis in non-conventional media (kinetics of biochemical reactions in organic solvents, supercritical fluids, two-phase systems: microemulsion and reverse micelles). Process development for obtaining optical pure compounds based on stereospecific biocatalysts. Enzyme deactivation in organic solvent. Lipase-catalyzed esterification, transesterification and hydrolysis in organic solvents). ;  Enzymatic synthesis and modification of bioactive compounds. Bioactive peptides syntheses by proteases. Examples for the application of oxidoreductases, lipases, esterases, aminoacylases, penicillin acylase and isomerases for production of bioactive compounds. ;  Optimization and control of reactions catalyzed by stereospecific enzymes. Examples of enatioselective syntheses for equilibrium and kinetically controlled reactions. Kinetic resolution or asymmetric synthesis of alcohols, esters, diols, amines, amides, peptides and others. Industrial biotransormations. Selected examples of biocatalytic syntheses of chiral intermediates for drugs. ;  Immobilized biocatalysts; optimization of process based on immobilized biocatalysts; productivity of immobilized systems; Examples for the application of immobilized enzymes for fine chemical and pharmaceutical syntheses. ;  Production of 6-aminopenicillanic acid (6-APK) and antibiotics with immobilized penicillin acylase: process design. Specific enzymes and their applications. ;
    Contents of exercisesThe Lab is design to equip the students with the necessary tools for obtaining and analyzing kinetic data effectively. Two-substrate esterification reactions in organic solvent catalyzed by lipase is used as a model reaction. Lab includs 1) Kinetics of lipase-catalyzed two-substrate esterification model; 2) Kinetics of penicillin acylase-catalyzed synthesis of an intermediate for semi-synthetic β-lactam; 3) Production of lipase by Aspergillus oryzae, purification and characterization; 4) Production of bioactive protein hydrolyzates and determination of their biological activities.
    1. R.A. Alberty, Enzyme kinetics, Wiley, 2011.
    2. Z. Knežević-Jugović, Enzimsko inženjerstvo, udžbenik, TMF, 2008.
    3. H.A. Kirst, W.K. Jeh, M.J. Zmijevski, Enzyme technologies for pharmaceutical and biotechnological applications, Marcel Dekker, 2001.
    4. A.S. Bommarius, B.R. Riebel, “Biocatalysis: fundamentals and applications, Wiley-VCH, 2004.
    5. A.G. Marangoni, Enyzme kinetics: a modern approach, John Wiley & Sons, Inc., Hoboken, New Jersey, 2003.
    Number of hours per week during the semester/trimester/year
    LecturesExercisesOTCStudy and ResearchOther classes
    Methods of teachingTheoretical lectures. Laboratory practice. Problem solving and calculus.
    Knowledge score (maximum points 100)
    Pre obligationsPointsFinal examPoints
    Activites during lecturesTest paper40
    Practical lessons20Oral examination