22MOPBI1 - Selected topics in biochemical engineering
| Course specification | ||||
|---|---|---|---|---|
| Course title | Selected topics in biochemical engineering | |||
| Acronym | 22MOPBI1 | |||
| Study programme | ||||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 4.0 | Status | ||
| Condition | Нема | Облик условљености | ||
| The goal | The aim of the course is to give the students more fundamentals and practical knowledge about general rules for optimization, modeling and design of enzymatic and other bioprocesses which are based on complex kinetics and application of free or immobilized enzymes, in aqueous and non-conventional media. Based on selected topics from biotechnological and pharmaceutical industry students will be able to implement both biochemical and engineering knowledge in order to design new and improve current biotechnological processes. | |||
| The outcome | After the course students will gain knowledge of basic principle of biocatalysis with sterospecific and immobilized biocatalysts in aqueous and non-conventional systems, which are of importance in actual biotechnological and pharmaceutical processes. They will be able to apply the theoretical knowledge to specific industrial bioprocess, predict the course of a biochemical process by kinetic calculation and design new processes in these complex systems. | |||
| Contents | ||||
| Contents of lectures | Kinetics of two-substrate reactions. Progress curve and determination of optimal kinetic models. The inhibition of two-substrate reactions: kinetic models. Mechanisms of drug actions by enzyme inhibition. Biocatalysis in non-conventional media (kinetics of biochemical reactions in orgaanic solvents, supercritical fluids, two-phase systems: microemulsion and reverse micelles). Lipase-catalyzed esterification, transesterification and hydrolysis in organic solvents. Specificity of biocatalyst; Analysis of enzyme enantiospecificity; Role of the substrate (alcohol moiety, acyl moiety); Role of the solvent and protein. Optimization and control of reactions catalyzed by stereospecific enzymes. Examples of enatioselective syntheses for equilibrium and kinetically controlled reactions. Process development for obtaining optical pure compounds based on stereospecific biocatalysts (kinetic resolution or asymmetric synthesis of alcohols, esters, diols, amines, amides, peptides and others). Biotechnological synthesis and modification of bioactive compounds. Bioactive peptides syntheses by proteases. Examples for the application of oxidoreductases, lipases, esterases, aminoacylases, penicillin acylase and isomerases for production of bioactive compounds. Industrial biotransformations with immobilized biocatalysts; optimization of process based on immobilized biocatalysts; Productivity of immobilized systems; Examples for the application of immobilized enzymes... | |||
| Contents of exercises | The Lab is design to equip the students with the necessary tools for obtaining and analyzing kinetic data effectively. Two-substrate esterification reactions in organic solvent catalyzed by lipase is used as a model reaction. Lab includes 1) Kinetics of lipase-catalyzed two-substrate esterification model; 2) Kinetics of penicillin acylase-catalyzed synthesis of an intermediate for semi-synthetic β-lactam antibiotics; 3) Enzymatic synthesis of prebiotic oligosaccharides, apllication of different bioseparation tecniques and instrumental analysis of their effects on purity of final product. | |||
| Literature | ||||
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| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 2 | 1 | 1 | ||
| Methods of teaching | Theoretical lectures. Laboratory practice. Problem solving and calculus. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | 40 | ||
| Practical lessons | 20 | Oral examination | ||
| Projects | ||||
| Colloquia | 25 | |||
| Seminars | 15 | |||