22FI41 - Enzyme engineering
| Course specification | ||||
|---|---|---|---|---|
| Course title | Enzyme engineering | |||
| Acronym | 22FI41 | |||
| Study programme | ||||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 5.0 | Status | ||
| Condition | Облик условљености | |||
| The goal | The course provides a strong background in enzyme technology and contemporary techniques including large-scale enzyme production, purification and characterization,immobilization of enzymes, procedures for obtaining novel industrial enzymes, and application of enzymes. In addition, the aim of the course is to provide students with theoretical knowledge and a range of academic skills in the optimization, modeling and design of bioprocesses that are based on the use of free and immobilized enzymes as catalysts. | |||
| The outcome | After the course, the students will gain knowledge of basic principles for optimization, modeling and design of bioprocesses in which both, free and immobilized enzymes play a role. By mastering enzyme purification and other techniques for enzyme assay and recovery as well as the numerical methods to analyze enzyme kinetic data, the students will have a strong background in different practical skills to formulate and solve problems in the field of enzyme technology. | |||
| Contents | ||||
| Contents of lectures | • An introduction in Enzyme technology and basic principles of biocatalysis; • Enzyme production (Enzyme sources: animal and plant enzymes; Industrial production of microbial enzymes; Specificity of enzyme production and regulation mechanisms of enzyme biosynthesis; Optimization and technology of industrial enzyme production; Application of gentic engineering); • Enzyme isolation, purification and characterization (General principles; methods for cell disruption; removal of whole cell and cell debris; Concentration and primary purification; Isolation of membrane-bound enzymes: Column chromatography; Evidence of purity; Scale-up and technological schemes of industrial enzymes` production; Legislative and safety aspects). • Immobilization of enzymes (Methods and supports; Effect of immobilization on enzyme properties and stability); • Design and modelling of enzyme processes ( Effect of process parameters on enzyme reaction rate; Inhibition of enzyme processes; Enzyme kinetics in homogeneous and heterogeneous systems with calculus). • Industrial enzymes: general characteristics and applications in pharmacy, medicine, analytics etc. (oxidoreductase, amylases; cellulases and hemicellulase, proteases, lipases; aminoacylase; penicillin acylase). | |||
| Contents of exercises | Calculus for modelling of enzyme processes. The Lab involves the production and isolation of peroxidase from horseradish, peroxidase purification using several chromatographic techniques, purity check by electrophoresis and HPLC chromatography. In addition, the exercises include isolating invertase from yeast and determining the kinetic parameters of the isolated enzyme by processing the data and applying various linearization methods of the Michaelis-Menten equation. The Lab is designed to provide the students with the necessary techniques for isolation, concentration and primary purification of enzyme such as cell rupture by several techniques, removal of cell debris by centrifugation, ultrafiltration, precipitation (solvent, salting out), enzyme purification in two-phase systems and column chromatography). Subsequently, students use isolated and purified enzymes for kinetic analyѕes. | |||
| Literature | ||||
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| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 2 | 1 | 2 | ||
| Methods of teaching | Teaching methodology consists of lectures with pronounced interactive character, tutorials, probleme solving and demonstrations. Lecturing will be accompanied by VIDEO BEAM presentation. The course is accompanied by coordinated lab exercises, include the work of students in small groups on modern equipment, measurement, tutorials collection, analysis, interpretation and presentation of kinetic data as well as discussion of given problems. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | 50 | ||
| Practical lessons | 30 | Oral examination | ||
| Projects | ||||
| Colloquia | 20 | |||
| Seminars | ||||