22BBI30 - Biotechnology lab 1
| Course specification | ||||
|---|---|---|---|---|
| Course title | Biotechnology lab 1 | |||
| Acronym | 22BBI30 | |||
| Study programme | Biochemical Engineering and Biotechnology | |||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 3.0 | Status | ||
| Condition | Облик условљености | |||
| The goal | The main goal is to enable students to use biochemical methods for identification and quantitative determination the most important biological molecules, which are prerequisite for biochemical processes monitoring. Students will be able to apply methods for determination of vitamins, carbohydrates and protein concentration; monitoring enzymatic transformation of biological molecules, performing enzyme activity assays and determining kinetic constants. | |||
| The outcome | SUpon the course completion students will be able to apply techniques and methods that are used in biotechnological laboratory. During the course students gain practical knowledge needed for detection and quantification of bioactive cell compounds (vitamins, proteins and DNA) and their further identification and characterization. Moreover, students will learn how to analyze, interpret and present experimental data. | |||
| Contents | ||||
| Contents of lectures | ||||
| Contents of exercises | Quantification of vitamin C using volumetric method. Determination of reducing sugars. Protein detection and quantitative determination with Lowry and/or Bradford method. Enzymatic hydrolysis of oligosaccharides and lipids. Spectroscopic enzyme activity assay. Determination of activity and specific activity of an enzyme for different substrates. Electrophoretic separation of proteins and DNA. Studying the effect of experimental conditions (pH, temperature, initial substrate and enzyme concentration) on enzyme activity. Kinetic constant calculation and graphical presentation of results using MS Excell. | |||
| Literature | ||||
| ||||
| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 0 | 1 | 3 | ||
| Methods of teaching | Laboratory practice. Experimental data analysis and calculus. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | 40 | ||
| Practical lessons | 20 | Oral examination | ||
| Projects | ||||
| Colloquia | 40 | |||
| Seminars | ||||