14MOBT - Selected bioanalytical techniques
| Course specification | ||||
|---|---|---|---|---|
| Course title | Selected bioanalytical techniques | |||
| Acronym | 14MOBT | |||
| Study programme | Biochemical Engineering and Biotechnology,Chemical engineering | |||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 4.0 | Status | ||
| Condition | Облик условљености | |||
| The goal | The main goal is to enable students to use the most important methods and techniques for identification, quantification and characterization of biomolecules. The most relevant methods for quantitative determination of biomolecules, used as substrates of biochemical processes or being products of the same, such as proteins, carbohydrates, lipids, nucleic acids and vitamins. | |||
| The outcome | Students will learn chromatographic and spectroscopic methods applied in quantitative analysis of biomolecules, as well as electrophoretic methods of biomolecules characterization. During the laboratory work these methods will be applied in following the course of particular bioprocesses, such as enzymatic reactions, fermentations and enzyme immobilizations. | |||
| Contents | ||||
| Contents of lectures | Chromatography of biomolecules. Plate theory of chromatography. van Deemter equation. Detectors and elution regimes. Ion exchange chromatography. Affinity chromatography. Gel permeation chromatography. Reversed-phase chromatography. Hydrophobic interaction chromatography. ; Quantification assays for biomolecules. Protein quantification assays. Carbohydrate quantification assays. Nucleic acid quantification assays. Lipid quantification assays. Vitamin quantification assays. ; Electrophoretic characterization of biomolecules. Electrophoretic support media. Detection of proteins and nucleic acids after electrophoretic separation. Zone electrophoresis. Isoelectric focusing. ; Validation of new bioanalytical methods. Precision and accuracy. Limits of quantitation and detection. ; | |||
| Contents of exercises | Spectroscopic assays for total sugars and reducing sugars. Glucose determination with spectroscopic assay using GOD-PAP reagent. Application of specific method for glucose determination in following the course of enzyme-catalyzes lactose hydrolysis and glucose isomerisation. Chromatografic determination of monosaccharides and oligosaccharides. ; Protein and nucleic acid assays. Following the course of protein immobilization and microbial enzyme production and electrophoretic characterization of final product. ; Chromatographic determination of vitamins and physiologically active compounds. Chromatographic determination of fatty acid composition of fats and oils. ; | |||
| Literature | ||||
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| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 1 | 2 | |||
| Methods of teaching | Theoretical lectures. Laboratory practice. Problem solving and calculus. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | 40 | ||
| Practical lessons | Oral examination | |||
| Projects | ||||
| Colloquia | 40 | |||
| Seminars | 20 | |||