14BBI471 - Metabolic and Genetic Engineering

Course specification
Course titleMetabolic and Genetic Engineering
Study programmeBiochemical Engineering and Biotechnology
Lecturer (for classes)
Lecturer/Associate (for practice)
Lecturer/Associate (for OTC)
    ConditionОблик условљености
    The goalThe aim of the course is that students acquire basic academic and scientific knowledge and practical experience in the area of metabolic and genetic engineering. which could be applied for the improvement and development of the cells and orgnisms used for various biotechnological purposes (food, diagnostics, medicine, ecology).
    The outcomeThe course prepares students to understand significance and applications of genetic and metabolic engineering and to master various techniques used in biotechnologies.
    Contents of lecturesBasic regulatory mechanisms of the cell metabolism, Techniques for the manipulations of the cell metabolism in order to improve the cell productivity in various biotechnological processes, Mutations, Basic techniques of genetic engineering: Isolation and purification of DNA, Electrophoresis, DNA cutting, Gene probes, Gene transfer, Gene vectors and its design, PCR technique, Genomics, Proteomics, Microarrays and DNA chips. Systems for the production of recombinant proteins. The application of genetic engineering in industry, plant production, medicine and pharmacy. Genetic instability as a problem in biotechnological processes and ways to overcome it.
    Contents of exercisesPractical lessons are based on laboratory practice of isolation of DNA from biological material, utilization of specific techniques of metabolic and genetic engineering and calculations.
    1. Ljiljana Mojović, Biohemijsko inženjerstvo, TMF, Beograd, 2006. (Original title)
    2. Michael L. Shuler, Fikret Kargi, Bioprocess engineering, Basic concepts, sec. edition. 2002, PHI, India (Original title)
    3. Wilson K., Walker J. Principles and techniques of Biochemistry and Molecular Biology, 2005. Cambridge University Press. (Original title)
    Number of hours per week during the semester/trimester/year
    LecturesExercisesOTCStudy and ResearchOther classes
    Methods of teachingInteractive lessons with using video presentations; biochemical and kinetic models and calculations; consultations
    Knowledge score (maximum points 100)
    Pre obligationsPointsFinal examPoints
    Activites during lectures5Test paper50
    Practical lessons5Oral examination