22BBI474 - Isolation and purification of biotechnological products
| Course specification | ||||
|---|---|---|---|---|
| Course title | Isolation and purification of biotechnological products | |||
| Acronym | 22BBI474 | |||
| Study programme | Biochemical Engineering and Biotechnology | |||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 4.0 | Status | ||
| Condition | Облик условљености | |||
| The goal | Objective of the course is to learn properties of biological compounds important for their separation, criteria for selection of separation technique, theoretical background of each method, applied equipment, and fields of application. The course will be organized in such a way that students will start with learning low resolution bioseparation techniques with further increase of resolution throughout semester and finishing with high resolution chromatographic techniques. | |||
| The outcome | During this course students will gain knowledge of basic principles and techniques for separation of cells, proteins, biopharmaceuticals and other fermentation products. After course students will be enabled to choose adequate separation procedure based on knowing characteristic properties of biological products. Problem solving (calculation and SuperPro Designer projects) will provide students opportunity to optimize the process of isolation of particular products. | |||
| Contents | ||||
| Contents of lectures | General and specific requests in bioseparation engineering. Classification of biological compounds and specific properties important for their separation. Cell disruption. Equipment, methods and selection criteria. Kinetics of intracellular compounds release. Solid-liquid separation. Sedimentation (coagulation and flocculation) and centrifugation (theoretical background, equipment, application, selection criteria). Filtration (process at constant pressure or constant flow, equipment, application in bioseparations). Crystallization, precipitation and adsorption (theoretical background, application). Extraction. Solid-liquid extraction (theoretical background, equipment, application). Liquid-liquid extraction (theoretical background, equipment, application). Supercritical fluid extraction (theoretical background, application). Bioseparation in aqueous two-phase systems (ATPS). Membrane processes. Microfiltration, ultrafiltration, nanofiltration, reverse osmosis. Electrodialysis. Membrane properties, membrane modules, process types (batch, continuous, diafiltration). Chromatography in bioseparations. Theoretical background (operating regime, columns, detectors, van Demter equation). Techniques (gel permeation chromatography, hydrophobic interaction chromatography, reversed phase chromatography, affinity chromatography, ion exchange chromatography, ion exclusion chromatography). | |||
| Contents of exercises | Practices. Calculation of cell release kinetics and selection of adequate disruption method. Adsorption of biological compounds and calculation of breakthrough curve. Calculation of centrifugation capacity. Calculation of liquid-liquid extraction and extraction in ATPS. Calculation of membrane processing efficiency in separation of biological compounds. Examples of various types of chromatography in bioseparations, calculation of separation effects and scale-up. LAB. Separation process integration and simulation using SuperPro Designer software. Laboratory work on membrane processing (diafiltration). | |||
| Literature | ||||
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| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 1 | 2 | 1 | ||
| Methods of teaching | Theoretical lectures. Problem solving and calculus. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | 40 | ||
| Practical lessons | 20 | Oral examination | ||
| Projects | ||||
| Colloquia | 40 | |||
| Seminars | ||||