22ZP313 - Biochemistry
| Course specification | ||||
|---|---|---|---|---|
| Course title | Biochemistry | |||
| Acronym | 22ZP313 | |||
| Study programme | Biochemical Engineering and Biotechnology | |||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 5.0 | Status | ||
| Condition | 1. and 2. min. 60 ECTS; 3. min. 40 ECTS; | Облик условљености | ||
| The goal | The objective is to enable students to understand basic metabolic processes of living cell, which comprise two key aspects - production of energy by substrate depletion and formation of building blocks for synthesis of cell constituents and microbal metabolites. Knowledge of biochemical processes is necessity for understanding and optimal performance of biotechnologицal processes based on biocatalytic activity of living cells. | |||
| The outcome | After the course students should be enabled to apply specific knowledge about biosynthesis of cell constituents and metabolites in development, optimization and control of actual biotechnological processes. Gained knowledge and information should enable students to develop strategic approach for solving problems of manufacture, storage and application of food, pharmaceutics and other products of biotechnology. | |||
| Contents | ||||
| Contents of lectures | Introduction to Biochemistry. Basic definitions (catabolism, anabolism, methabolic pathways, main reaction classes of biochemistry). Classification of organisms (based on energy source, electron donor and final acceptor). Bioenergetics. Standard and physiological free-energy change. Coupled reactions. High-energy compounds. Carbohydrate catabolism. Glycolysis (EMP pathway). Pentose phosphate pathway (HMP shunt). Entner-Doudoroff (ED) pathway. Phosphoketolase pathway. Citric acid cycle. Glyoxylate cycle. Catabolism of polysaccharides, other hexoses and uronic acids. Respiratory (electron transport) chain. Biological redox reactions. Biological electron carriers. Mitchell's chemiosmotic theory and role of ATP synthase. P/O ratio. Carbohydrate anabolism. Gluconeogenesis. Glycogenesis. Photosynthesis (light-dependent reactions and Calvin cycle). Lipid catabolism. Lipid hydrolysis. β-oxidation of fatty acids. Energy yield of fatty acid oxidation. Lipid anabolism. Biosynthesis of saturated and unsaturated fatty acids. Biosynthesis of glycerol and lipids. Regulation of fatty acid metabolism. Protein catabolism. Protein hydrolysis. Transamination and deamination of amino acids. Urea cycle. Amino acid degradation pathways.Protein anabolism. Nitrogen cycle. Incorporation of ammonia in biomolecules. Biosynthesis of amino acids. Biosynthesis of molecules derived from amino acids. Nucleic acid metabolism. Hydrolysis of nucleic acids. Degradation of purine and pyrimidine nucleotides. | |||
| Contents of exercises | Biological role of uronic acids and amino sugars in cell recognition and synthesis of polysaccharides. The role of lipoproteins in transport of lipid degradation products (definition of LDL and HDL particles). The role of gluthation in prevention of oxidative damage of cell proteins and nucleic acids. Biosynthesis of melanine and heme and diseases caused by deffects in biosynthetic pathways. Inhibitors of nucleotide biosynthesis as therapeutics. Competitive or irreversible enzyme inhibitors as therapeutics. | |||
| Literature | ||||
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| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 3 | 1 | |||
| Methods of teaching | Theoretical lectures and practices focused on definition of biological roles of specific molecules. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | |||
| Practical lessons | Oral examination | 70 | ||
| Projects | ||||
| Colloquia | 30 | |||
| Seminars | ||||