22BBI472 - Enzyme engineering
| Course specification | ||||
|---|---|---|---|---|
| Course title | Enzyme engineering | |||
| Acronym | 22BBI472 | |||
| Study programme | Biochemical Engineering and Biotechnology | |||
| Module | ||||
| Lecturer (for classes) | ||||
| Lecturer/Associate (for practice) | ||||
| Lecturer/Associate (for OTC) | ||||
| ESPB | 6.0 | Status | ||
| Condition | Облик условљености | |||
| The goal | The course provides a strong background to enzyme technology and contemporary techniques including enzyme kinetics and modelling of enzymatic reactions, large-scale enzyme production and purification, the application of recombinant DNA technology in enzyme production, procedures for obtaining novel industrial enzymes, immobilization of enzymes, as well as basic principles for optimization and design of enzymatic processes. | |||
| The outcome | After the course, the students will gain knowledge of basic principles for optimization, modeling and design of bioprocesses in which both, free and immobilized enzymes play a role. By mastering enzyme purification and other techniques for enzyme assay and recovery as well as the numerical methods to analyze enzyme kinetic data with the advance of a computer, the students will have a strong background in different practical skills to formulate and solve problems in the field of enzyme technology. | |||
| Contents | ||||
| Contents of lectures | • An introduction in Enzyme technology and basic principles of biocatalysis; • Enzyme production (Enzyme sources: animal and plant enzymes; Industrial production of microbial enzymes; Specificity of enzyme production and regulation mechanisms of enzyme biosynthesis; Optimization and technology of industrial enzyme production; Application of genetic engineering); • Enzyme isolation, purification and characterization (General principles; methods for cell disruption; removal of whole cell and cell debris; Concentration and primary purification; Isolation of membrane-bound enzymes; Column chromatography; Scale-up and technological schemes of industrial enzymes` production with calculus; Evidence of purity; Legislative and safety aspects; • Optimization of industrial enzymes by molecular engineering; • Immobilization of enzymes (Methods and supports; Properties of immobilized enzymes with calculus); • Optmization of enzymatic process (Influence of process parameters on the rate of enzymatic reactions, Chemical control of enzymatic reactions; • Design and modelling of enzyme processes (Enzyme reactors; Enzyme kinetics in homogeneous and heterogeneous systems with calculus); • Industrial enzymes: general characteristics and applications: 1) Oxidoreductase;2) Amylases and their application in starch industry;3) Pectinase;4) Cellulases and hemicellulase,5) Proteases and their application in detergent industry, food industry, chemical and textilе industry;6) Other enzymes: ... | |||
| Contents of exercises | Calculus from enzyme purification; modelling of enzymatic processes and enzyme immobilization. Labs includes a series of practical exercises: 1) Isolation of invertase from yeast and its partial purification (salting out, precipitation with organic solvents, precipitation on pI); 2) Isolation peroxidase from horseradish (extraction, salting out, ultrafiltration, dialysis, chromatographic methods); 3) Determination of molecular weight of isolated and purified peroxidase using permeable chromatography; 4) Purity check and determination of molecular weight of isolated peroxidase by SDS-PAGE electrophoresis; 5) Production of a-amylase, protease, pectinase and cellulase by solid-state fermentation; 6) Hydrolysis of soy proteins by proteolytic enzymes (determination of the degree of hydrolysis by several methods); 7) Separation of soy protein hydrolyzates into fractions using membrane separation techniques and determination of antioxidant activity of farctions; 8) Immobilization of peroxidase from horseradish by adsorption and determination of specific activity of the biocatalyst; 9) Immobilization of lipase by covalent method and determination of specific activity; 10) Enzymatic hydrolysis of starch and determination of dextrose equivalent. The Lab is designed to provide the students with the necessary techniques for isolation, concentration and primary purification of enzyme such as cell disintegration techniques, removal of cell debris by centrifugation, ultrafiltration... | |||
| Literature | ||||
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| Number of hours per week during the semester/trimester/year | ||||
| Lectures | Exercises | OTC | Study and Research | Other classes |
| 2 | 1 | 2 | ||
| Methods of teaching | Teaching methodology consists of lectures with pronounced interactive character, tutorials, probleme solving and demonstrations. Lecturing will be accompanied by VIDEO BEAM presentation. The course is accompanied by coordinated lab exercises, including measurement, tutorials collection, analysis, interpretation and presentation of data. | |||
| Knowledge score (maximum points 100) | ||||
| Pre obligations | Points | Final exam | Points | |
| Activites during lectures | Test paper | 40 | ||
| Practical lessons | 20 | Oral examination | ||
| Projects | ||||
| Colloquia | 40 | |||
| Seminars | ||||