Navigation

22BBI471 - Metabolic and Genetic Engineering

Course specification
Course titleMetabolic and Genetic Engineering
Acronym22BBI471
Study programmeBiochemical Engineering and Biotechnology
Module
Lecturer (for classes)
    Lecturer/Associate (for practice)
      Lecturer/Associate (for OTC)
        ESPB4.0Status
        ConditionОблик условљености
        The goalThe aim of the course is that students acquire basic academic and scientific knowledge and practical experience in the area of metabolic and genetic engineering. which could be applied for the improvement and development of the cells and orgnisms used for various biotechnological purposes (food, diagnostics, medicine, ecology).
        The outcomeThe course prepares students to understand significance and applications of genetic and metabolic engineering and to master various techniques used in biotechnologies.
        Contents
        Contents of lecturesBasic regulatory mechanisms of the cell metabolism, Techniques for the manipulations of the cell metabolism in order to improve the cell productivity in various biotechnological processes, Mutations, Basic techniques of genetic engineering: Isolation and purification of DNA, Electrophoresis, DNA cutting, Gene probes, Gene transfer, Gene vectors and its design, PCR technique, Genomics, Proteomics, Microarrays and DNA chips. Systems for the production of recombinant proteins. The application of genetic engineering in industry, plant production, medicine and pharmacy. Genetic instability as a problem in biotechnological processes and ways to overcome it.
        Contents of exercisesPractical lessons are based on laboratory practice of isolation of DNA from biological material, utilization of specific techniques of metabolic and genetic engineering and calculations.
        Literature
        1. Mojović Ljiljana, Biohemijsko inženjerstvo, TMF Beograd, 2006
        2. Michael L. Shuler, Fikret Kargi, Bioprocess engineering, Basic concepts, sec. edition. 2002, PHI, India
        3. Wilson K., Walker J. Principles and techniques of Biochemistry and Molecular Biology, 2005. Cambridge University Press.
        Number of hours per week during the semester/trimester/year
        LecturesExercisesOTCStudy and ResearchOther classes
        21
        Methods of teachingInteractive lessons with using video presentations; biochemical and kinetic models and calculations; consultations
        Knowledge score (maximum points 100)
        Pre obligationsPointsFinal examPoints
        Activites during lectures5Test paper50
        Practical lessons5Oral examination
        Projects
        Colloquia30
        Seminars10